Recent acquisitions in the pathophysiology, diagnosis and treatment of disseminated intravascular coagulation
© Franchini et al. 2006
Received: 03 January 2006
Accepted: 21 February 2006
Published: 21 February 2006
Disseminated intravascular coagulation (DIC) is a disorder characterized by both acute generalized, widespread activation of coagulation, which results in thrombotic complications due to the intravascular formation of fibrin, and diffuse hemorrhages, due to the consumption of platelets and coagulation factors. Systemic activation of coagulation may occur in a variety of disorders, including sepsis, severe infections, malignancies, obstetric or vascular disorders, and severe toxic or immunological reactions.
In this review, we briefly report the present knowledge about the pathophysiology and diagnosis of DIC. Particular attention is also given to the current standard and experimental therapies of overt DIC.
Clinical conditions associated with disseminated intravascular coagulation.
Gram-positive bacteria, Gram-negative bacteria, spirochetes, rickettsiae, protozoa, fungi, viruses
Polytrauma, neurotrauma, fat embolism, burns
Solid tumors, myeloproliferative/lymphoproliferative malignancies
Amniotic fluid embolism, abruptio placentae, placenta previa, retained dead fetus syndrome
Large vascular aneurysms, Kasabach-Merritt syndrome
Severe pancreatitis, severe hepatic failure
Snake bites, recreational drugs
Hemolytic transfusion reaction, transplant rejection
In the following sections we briefly discuss the current knowledge on the pathogenesis, diagnosis and treatment of DIC.
Pathogenesis of DIC
Most of the recent advances in our understanding ofthe pathogenesis of DIC are derived from studies in animal models and humans with severe sepsis . These studies have demonstrated that the systemic formation of fibrin observed in this setting is the result of the simultaneous coexistence of four different mechanisms: increased thrombin generation, a suppression of the physiologic anticoagulant pathways, impaired fibrinolysis and activation of the inflammatory pathway [1, 8, 21].
The systemic generation of thrombin has been shown to be mediated predominantlyby the extrinsic (factor VIIa) pathway. In fact, while the abrogation of the tissue factor/factor VIIa pathway resulted in complete inhibition of thrombin generation in experimental animal models of endotoxemia, the inhibition of the contact system did not prevent systemic activation of coagulation [22, 23].
Impaired function of physiological anticoagulant pathways may amplify thrombin generation and contribute to fibrin formation . Plasma levels of antithrombin are markedly reduced in septic patients as a result of a combination of increased consumption by the ongoing formation of thrombin, enzyme degradation by elastase released from activated neutrophils, impaired synthesis due to liver failure and vascular capillary leakage [6, 7, 25]. Likewise, there may be significant depression of the protein C system, caused by enhanced consumption, impaired liver synthesis, vascular leakage and a down-regulation of thrombomodulin expression on endothelial cells by pro-inflammatory cytokines, such as tumor necrosis factor (TNF)-α and interleukin(IL)-1β [26–28]. Moreover, the evidence that administration of recombinant tissue factor pathway inhibitor (TFPI) results in complete inhibition of endotoxin-induced thrombin generation suggests that tissue factor is involved in the pathogenesis of DIC [29, 30]. Although no acquired or deficiency or functional defect of TFPI has been identified in patients with DIC, there is evidence that the inhibitor does not regulate tissue factor activity sufficiently in such patients.
As regards impaired fibrinolysis, experimental models of bacteremia and endotoxemia are characterized by rapidly increasing fibrinolytic activity, most probably due to the release of plasminogen activators from endothelial cells. However, this initialhyperfibrinolytic response is followed by an equally rapid suppression of fibrinolytic activity, due to the increase in plasma levels of plasminogen activator inhibitor type 1 (PAI-1) [31–33]. The importance of PAI-1 in the pathogenesis of DIC is further demonstrated by the fact that a functional mutation in the PAI-1 gene, the 4G/5G polymorphism, which causes increased plasma levels of PAI-1, was linked to a worse clinical outcome in patients with meningococcal septicemia .
Finally, another important mechanism in the pathogenesis of DIC is the parallel and concomitant activation of the inflammatory cascade mediated by activated coagulation proteins, which in turn can stimulate endothelial cells to synthesize pro-inflammatory cytokines. In fact, while cytokines and inflammatory mediators can induce coagulation, thrombin and other serine proteasesinteract with protease-activated receptors on cell surfaces to promote further activation and additional inflammation . Furthermore, since activated protein C has an anti-inflammatory effect through its inhibition of endotoxin-induced production of TNF-α, IL-1β, IL-6 and IL-8 by cultured monocytes/macrophages, depression of the protein C system may result in a pro-inflammatory state .
Pathogenesis of disseminated intravascular coagulation in sepsis.
1) Increased thrombin generation
Mediated predominantlyby tissue factor/factor VIIa pathway
2) Impaired function of physiological anticoagulant pathway
a) Reduction of antithrombin levels
The result of a combination of increased consumption, enzyme degradation, impaired liver synthesis and vascular leakage
b) Depression of protein C system
The result of a combination of increased consumption, impaired liver synthesis, vascular leakage and down- regulation of thrombomodulin
c) Insufficienttissue factor pathway inhibitor (TFPI)
3) Impaired fibrinolysis
Mediated by release of plasminogen activators from endothelial cells immediately followed by an increase in the plasma levels of plasminogen activator inhibitor type 1 (PAI-1)
4) Activation of inflammatory pathway
Mediated by activated coagulation proteins and by depression of the protein C system
However, there is evidence that various events, including the release of tissue material (fat, phospholipids, cellular enzymes) into the circulation, hemolysis and endothelial damage may promote the systemic activation of coagulation in severe trauma and burns through a mechanism similar to that observed in septic patients (i.e., systemic activation of cytokines)[14, 15]. Nevertheless, there may also be specific variations in the pathogenesis if DIC due to different underlying disorders. For example, in some patients with cancer the initiation of coagulation activation is not only due to tissue factor expression on the surface of the malignant cells but in this case also involves a specific cancer procoagulant, a cysteine protease with factor X activating properties. Patients with acute promyelocytic leukemia have a peculiar form of DIC characterized by a severe hyperfibrinolytic state associated with systemic activation of coagulation.
Diagnosis of DIC
Treatment of DIC
Treatment modalities for disseminated intravascular coagulation.
1) Replacement therapy
- Fresh-frozen plasma
- Unfractionated and low-molecular-weight heparin
- Danaparoid sodium
- Recombinant hirudin
- Recombinant tissue factor pathway inhibitor
- Recombinant nematode anticoagulant protein c2
3) Restoration of anticoagulant pathways
- Recombinant human activated protein C
4) Other agents
- Recombinant activated factor VII
- Antifibrinolytic agents
- Antiselectin antibodies
- Recombinant interleukin-10
- Monoclonal antibodies against TNF and CD14
a) Replacement therapy
The aim of replacement therapy in DIC is to replace the deficiency due to the consumptionof platelets, coagulation factors and inhibitors in order to prevent or arrest the hemorrhagic episodes . Platelet concentrates and fresh frozen plasma (FFP) were, in the past, used very cautiously because of the fear that they might "feed the fire" and worsen thrombosis in patients with active DIC. However, this fear was not confirmed by clinical practice and nowadays replacement therapy is a mainstay of the treatment of patients with significant bleeding and coagulation parameters compatible with DIC. Transfusion of platelet concentrates at 1–2 U/10 Kg body weight should be considered when the platelet count is less than 20 × 109/L or if there is major bleeding and the platelet count is less than 50 × 109/L. When there is significant DIC-associated bleeding and fibrinogen levels are below 100 mg/dL, the use of FFP, at a dose of 15–20 mL/Kg, is justified. Alternatively, fibrinogen concentrates (total dose 2–3 g) or cryoprecipitates (1 U/10 Kg body weight) may be administered. However, FFP should be preferred to specific coagulation factor concentrates since the former contains all coagulation factors and inhibitors deficient during active DIC and lacks traces of activated coagulation factors, which may instead contaminate the concentrates and exacerbate the coagulation disorder.
The role of heparin in the treatment of DIC remains controversial [55–62]. In fact, although from a theoretical point of view interruption of the coagulation cascade should be of benefit in patients with active DIC , the clinical studies carried out so far have not been conclusive and indeed have often yielded contradictory results . However, on the basis of the few data available in the literature, heparin treatment is probably useful in patients with acute DIC and predominant thromboembolism, such as those with purpura fulminans [2, 56]. The use of heparin in chronic DIC is better established and it has been successfully employed in patients with chronic DIC associated with those diseases in which recurrent thrombosis predominates, such as solid tumors, hemangiomas, and dead fetus syndrome . The role of heparin in the treatment of DIC associated with acute promyelocytic leukemia (APL) is another controversy, since some authors support its use whereas other studies failed to demonstrate its efficacy [57–59]. However, the use of heparin in this setting has declined in the last few years thanks to the introduction of all-trans retinoic acid therapy which has led to the reduction of APL-associated coagulopathy. Heparin is usually given at relatively low doses (5–10 U/Kg of body weight per hour) by continuous intravenous infusion and may be switched to subcutaneous injection for long-term outpatient therapy (i.e. for those patients with chronic DIC associated with solid tumors). Alternatively, low-molecular-weight heparin may be used, as supported by the positive results in both experimental and clinical DIC studies [60–63].
A newer anticoagulant agent with direct thombin inhibitory activity, recombinant hirudin (r-hirudin) [67, 68], was recently shown to be effective in treating DIC in animal studies, although a study of the effect of this thrombin inhibitor in a sheep model of lethal endotoxemia showed no benefit . Preliminary experimental human studies proved that this drug attenuated endotoxin-induced coagulation activation .
Since activation of the coagulation cascade during DIC occurs predominantlythrough the extrinsic pathway, theoretically the inhibition of tissue factor should block endotoxin-associated thrombin generation . In vivo experiments in baboon models of lethal DIC showed that TFPI is a potent inhibitor of sepsis-related mortality . De Jonge and colleagues  first demonstrated in humans that recombinant TFPI dose-dependently inhibits coagulation activation during endotoxemia. Recombinant TFPI was evaluated in a phase II randomized trial in patients with severe sepsis . Although the study did not have the statistical power to demonstrate a survival benefit, it did show a trend toward a reduction in 28-day all-cause mortality together with an improvement in organ dysfunction in the group of patients treated with the recombinant TFPI. No evidence of a survival advantage was observed in patients with severe sepsis who received recombinant TFPI in a recent phase III large clinical trial.
Inhibition of the tissue factor/factor VIIa pathway is an another strategy that has been explored. Moons and colleagues demonstrated the efficacy of recombinant nematode anticoagulant protein c2 (NaPc2), a potent and specific inhibitor of the ternary complex between tissue factor/factor VIIa and factor Xa, in inhibiting coagulation activation in a primate model of sepsis . Other authors have experimented with anti-tissue factor/factor VIIa antibodies in animal models with promising results .
c) Restoration of anticoagulant pathways
Since patients with active DIC have an acquired deficiency of coagulation inhibitors, restoration of the physiologic anticoagulation pathways seems to be an appropriate aim of the treatment of DIC . Considering that antithrombin (AT) is the primary inhibitor of circulating thrombin, its use in DIC is certainly rational . Recent studies in animals and humans with severe sepsis have demonstrated that antithrombin also has anti-inflammatory properties (reduction of C-reactive protein and IL-6 levels), which may further justify its utilization during DIC [78, 79]. The administration of antithrombin concentrates infused at supraphysiologic concentrations was shown to reduce sepsis-related mortality in animal models . Several small clinical trials have been conducted in humans, mostly in patients with sepsis-related DIC, and have shown beneficial effects in terms of improvement of coagulation parameters and organ function [81, 82]. An Italian multicenter, randomized, double-blind study conducted in 1998 by Baudo et al. , evaluating the role of antithrombin in patients with sepsis or post-surgical complications, showed a net beneficial effect on survival in those patients receiving the concentrate. These findings were confirmed in 1999 by Levi et al. in their meta-analysis of all so far published human clinical trials of antithrombin treatment of DIC . By contrast, a large randomized, controlled multicenter trial of supraphysiologic doses of AT concentrates conducted in 2144 patients with sepsis and DIC did not show a beneficial effect of antithrombin treatment . However, a retrospective analysis of the same trial showed that the subgroup of patients who did not receive concomitant heparin had a potential benefit from antithrombin III in terms of mortality reduction.
Based on the fact that the protein C system is impaired during DIC some authors have investigated the therapeutic efficacy of exogenous administration of this protein in patients with DIC [86–98]. The infusion of activated protein C (APC) concentrates was shown to prevent DIC and mortality in an animal model of sepsis . A study conducted in 1998 on patients with severe sepsis suggested a trend toward improved survival in the group treated with APC . In a dose-ranging clinical trial, 131 patients with sepsis received recombinant human APC by continuous infusion at doses ranging from 12μg/Kg/hour to 30 μg/Kg/hour or placebo . A 40 percent reduction in mortality was observed in those patients who received the higher doses of activated protein C. Similarly, another recent multicenter clinical trial  determined that treatment with recombinant human APC, given intravenously at a dose of 24 μg/Kg of body weight per hour, significantly reduced mortality in patients with severe sepsis, in spite of a higher rate of serious bleeding in the APC-treated group. A double-blind randomized trial compared the safety and efficacy of APC and unfractionated heparin in the treatment of DIC and concluded that the former improved DIC, and finally the survival, more efficiently than did heparin . The recently published results of the trial conducted by the Human Recombinant Activated Protein C Worldwide Evaluation in Sepsis (PROWESS) Study Group showed a significant reduction in 28-day mortality and a quicker resolution of organ dysfunction in the group of patients with severe sepsis treated with APC [92, 93]. These results were confirmed by the ENHANCE trial which also suggested that recombinant APC might be more effective is therapy is started earlier . By contrast, a very recent trial on 2640 patients with severe sepsis and a low risk of death (defined by an Acute Physiology and Chronic Health Evaluation [APACHE II] score <25 or single organ failure) did not find a statistically significant difference in 28-day mortality rate between the placebo and APC-treated groups, suggesting that APC is of benefit only in patients at high risk of death from sepsis.Ongoing studies are focusing on the concomitant use of heparin in patients with DIC who receive activated protein C .
d) Other agents
Recombinant factor VII activated (rFVIIa) may be used in patients with severe bleeding who are not responsive to other treatment options. Bolus doses of 60–120 μg/Kg, possibly repeated after 2–6 hours, have been found to be effective in controlling refractory hemorrhagic episodes associated with DIC [100, 101]. Antifibrinolytic agents, such as epsilon-aminocaproic acid or tranexamic acid, given intravenously at a dose of 10–15 mg/Kg/h, are occasionally used in patients resistant to replacement therapy who are bleeding profusely or in patients with disease states associated with intense fibrinolysis (prostate cancer, Kasabach-Merrit syndrome, acute promyelocytic leukemia) . However, since these agents are very effective in blocking fibrinolysis, they should not be administered unless heparin has been previously infused in order to block the prothrombotic component of DIC. The use of these drugs in APL has declined in the last few years, given the efficacy of all-trans-retinoic-acid in preventing the majority of the hemorrhagic complications of this malignancy .
The advances in the understanding of the pathophysiology of DIC have resulted in novel preventive and therapeutic approaches to this disease. Based on the fact that tissue inflammation is a fundamental mechanism in DIC associated with sepsis or major trauma, some researchers have successfully employed the combined blockade of leukocyte/platelet adhesion and coagulation in a murine model by using antiselectin antibodies and heparin and have suggested the potential clinical use of such a strategy . Based on the same rationale, other researchers have demonstrated that the administration of recombinant IL-10, an anti-inflammatory cytokine which may modulate the activation of coagulation, completely abrogated endotoxin-induced effects on coagulation in humans . By contrast, the use of monoclonal antibodies against tumor necrosis factor has shown disappointing or at best modest results in septic patients[105–107]. More recently, Branger and colleaguesfound that an inhibitor of p38 mitogen-activated protein kinase (MAPK), an important component of intracellular signaling cascades that mediate the inflammatory response to infectious and non-infectious stimuli, attenuated the activation of coagulation, fibrinolysis and endothelial cells during human endotoxemia. Finally, although studies using antibodies against the receptor for bacterial endotoxins (CD14) produced positive results, other studies using endotoxin antibodies failed to improve outcome[110, 111].
Disseminated intravascular coagulation is a syndrome characterized by systemic intravascular activation of coagulation leading to bleeding (due to depletion of platelets and coagulation factors) and thrombosis(due to widespread deposition of fibrin in the circulation).
The diagnosis of DIC is usually made by a combination of routinely available laboratory tests, using a validated diagnostic algorithm.
In recent years, the mechanisms involved in pathological microvascular fibrin deposition in DIC have become progressively clear, resulting in novel preventive and therapeutic approaches to patients with DIC.
- Bick RL: Disseminated intravascular coagulation. Current concepts of etiology, pathophysiology, diagnosis, and treatment. Hematol Oncol Clin N Am 2003, 17:149–176.View Article
- Levi M, ten Cate H: Disseminated intravascular coagulation. N Engl J Med 1999, 341:586–592.PubMedView Article
- Levi M, de Jonge E, Van der Poll T, ten Cate H: Disseminated intravascular coagulation: state of the art. Thromb Haemost 1999, 82:695–705.PubMed
- Levi M, de Jonge E, Meijers J: The diagnosis of disseminated intravascular coagulation. Blood Rev 2002, 16:217–223.PubMedView Article
- Mammen EF: Disseminated intravascular coagulation (DIC). Clin Lab Sci 2000, 13:239–245.PubMed
- Levi M: Disseminated intravascular coagulation: what's new? Crit Care Clin 2005, 21:449–467.PubMedView Article
- Carey MJ, Rogers GM: Disseminated intravascular coagulation: clinical and laboratory aspects. Am J Hematol 1998, 59:65–73.PubMedView Article
- Levi M: Current understanding of disseminated intravascular coagulation. Br J Haematol 2004, 124:567–576.PubMedView Article
- Faust SN, Heyderman RS, Levin M: Disseminated intravascular coagulation and purpura fulminans secondary to infection. Best Pract Res Clin Haematol 2000, 13:179–197.View Article
- Wheeler AP, Bernard GR: Treating patients with severe sepsis. N Engl J Med 1999, 340:207–214.PubMedView Article
- Bone RC: Gram-positive organisms and sepsis. Arch Intern Med 1994, 154:26–34.PubMedView Article
- Dempfle C-E: Coagulation of sepsis. Thromb Haemost 2004, 91:213–224.PubMed
- Amaral A, Opal SM, Vincent J-L: Coagulation in sepsis. Intensive Care Med 2004, 30:1032–1040.PubMedView Article
- Roumen RM, Hendriks T, van der Ven-Jongekrijg J, et al.: Cytokine patterns in patients after major vascular surgery, hemorrhagic shock, and severe blunt trauma. Relation with subsequent adult respiratory distress syndrome and multiple organ failure. Ann Surg 1993, 218:769–776.PubMedView ArticlePubMed Central
- Gando S: Disseminated intravascular coagulation in trauma patients. Semin Thromb Hemost 2001, 27:585–592.PubMedView Article
- Colman RW, Rubin RN: Disseminated intravascular coagulation due to malignancy. Semin Oncol 1990, 17:172–186.PubMed
- Donati MB, Falanga A: Pathogenetic mechanisms of thrombosis in malignancy. Acta Haematol 2001, 106:18–24.PubMedView Article
- Barbui T, Falanga A: Disseminated intravascular coagulation in acute leukemia. Semin Thromb Hemost 2001, 27:593–604.PubMedView Article
- Letsky EA: Disseminated intravascular coagulation. Best Pract Res Clin Obstet Gynaecol 2001, 4:623–644.View Article
- Weiner CP: The obstetric patient and disseminated intravascular coagulation. Clin Perinatol 1986, 13:705–717.PubMed
- Levi M, de Jonge E, van der Poll T: Advances in the understanding of the pathogenetic pathways of disseminated intravascular coagulation result in more insight in the clinical picture and better management strategies. Semin Thromb Hemost 2001, 27:569–575.PubMedView Article
- Pixley RA, De La Cadena R, Page JD, et al.: The contact system contributes to hypotension but not disseminated intravascular coagulation in lethal bacteremia. In vivo use of a monoclonal anti-factor XII antibody to block contact activation in baboons. J Clin Invest 1993, 91:61–68.PubMedView ArticlePubMed Central
- Biemond BJ, Levi M, ten Cate H, et al.: Complete inhibition of endotoxin-induced coagulation activation in chimpanzees with a monoclonal Fab fragment against factor VII/VIIa. Thromb Haemost 1995, 73:223–230.PubMed
- Esmon CT: The regulation of natural anticoagulant pathways. Science 1987, 235:1348–1352.PubMedView Article
- Seitz R, Wolf M, Egbring R, Havemann K: The disturbance of hemostasis in septic shock: role of neutrophil elastase and thrombin, effects of antithrombin III and plasma substitution. Eur J Haematol 1989, 43:22–28.PubMedView Article
- Conway EM, Rosenberg RD: Tumor necrosis factor suppresses transcription of the thrombomodulin gene in endothelial cells. Mol Cell Biol 1988, 8:5588–5592.PubMedPubMed Central
- Esmon CT: Role of coagulation inhibitors in inflammation. Thromb Haemost 2001, 86:51–56.PubMed
- Faust SN, Levi M, Harrison OB, et al.: Dysfunction of endothelial protein C activation in severe meningococcal sepsis. N Engl J Med 2001, 345:408–416.PubMedView Article
- De Jonge E, Dekkers PE, Creasey AA, et al.: Tissue factor pathway inhibitor dose-dependently inhibits coagulation activation without influencing the fibrinolytic and cytokine response during human endotoxemia. Blood 2000, 95:1124–1129.PubMed
- Creasey AA, Chang AC, Feigen L, Wun TC, Taylor FB Jr, Hinshaw LB: Tissue factor pathway inhibitor reduces mortality from Escherichia coli septic shock. J Clin Invest 1993, 91:2850–2860.PubMedView ArticlePubMed Central
- Levi M, van der Poll T, de Jonge E, ten Cate H: Relative insufficiency of fibrinolysis in disseminated intravascular coagulation. Sepsis 2000, 3:103–109.
- Biemond BJ, Levi M, Ten Cate H, et al.: Plasminogen activator and plasminogen activator inhibitor I release during experimental endotoxemia in chimpanzees: effect of interventions in the cytokine and coagulation cascades. Clin Sci 1995, 88:587–594.PubMed
- Suffredini AF, Harpel PC, Parrillo JE: Promotion and subsequent inhibition of plasminogen activation after administration of intravenous endotoxin to normal subjects. N Engl J Med 1989, 320:1165–1172.PubMedView Article
- Hermans PW, Hibberd ML, Booy R, Daramola O, Hazelzet JA, de Groot R, Levin M: 4G/5G promoter polymorphism in the plasminogen-activator-inhibitor-1 gene and outcome of meningococcal disease. Meningococcal Research Group. Lancet 1999, 354:556–560.PubMedView Article
- van der Poll T, de Jonge E, Levi M: Regulatory role of cytokines in disseminated intravascular coagulation. Semin Thromb Hemost 2001, 27:639–651.PubMedView Article
- Yuksel M, Okajima K, Uchiba M, Horiuchi S, Okabe H: Activated protein C inhibits lipopolysaccharide-induced tumor necrosis factor-alpha production by inhibiting activation of both nuclear factor-kappa B and activator protein-1 in human monocytes. Thromb Haemost 2002, 88:267–273.PubMed
- van der Poll T, Buller HR, ten Cate H: Activation of coagulation after administration of tumor necrosis factor to normal subjects. N Eng J Med 1990, 322:1622–1627.View Article
- Rickles FR, Falanga A: Molecular basis for the relationship between thrombosis and cancer. Thromb Res 2001, 102:V215–224.PubMedView Article
- Bick RL: Disseminated intravascular coagulation: objective clinical and laboratory diagnosis, treatment and assessment of therapeutic response. Semin Thromb Haemost 1996, 22:69–88.View Article
- Rocha E, Paramo JA, Montes R, Panizo C: Acute generalized, widespread bleeding. Diagnosis and management. Haematologica 1998, 83:1024–1037.PubMed
- Yu M, Nardella BS, Pechet L: Screening tests of disseminated intravascular coagulation: guidelines for rapid and specific laboratory diagnosis. Crit Care Med 2000, 28:1777–1780.PubMedView Article
- Wada H, Wakita Y, Nakase T, et al.: Increased plasma-soluble fibrin monomer levels in patients with disseminated intravascular coagulation. Am J Hematol 1996, 51:255–260.PubMedView Article
- Bauer KA, Rosenberg RD: The pathophysiology of the prethrombotic state in humans: insights gained from studies using markers of hemostatic system activation. Blood 1987, 70:343–350.PubMed
- Wada H, Gabazza EC, Asakura H, et al.: Comparison of diagnostic criteria for disseminated intravascular coagulation (DIC): diagnostic criteria of the International Society of Thrombosis and Hemostasis and of the Japanese Ministry of Health and Welfare for overt DIC. Am J Hematol 2003, 74:17–22.PubMedView Article
- Taylor FBJ, Toh CH, Hoots WK, Wada H, Levi M, Scientific Subcommittee on Disseminated Intravascular Coagulation (DIC) of the International Society of Thrombosis and Haemostasis (ISTH): Towards definition, clinical and laboratory criteria, and a scoring system for disseminated intravascular coagulation. Thromb Haemost 2001, 86:1327–1330.PubMed
- Bakhtiari K, Meijers JCM, de Jonge E, Levi M: Prospective validation of the International Society of Thrombosis and Haemostasis scoring system for disseminated intravascular coagulation. Crit Care Med 2004, 32:2416–2421.PubMedView Article
- Toh CH, Downey C: Performance and prognostic importance of a new clinical and laboratory scoring system for identifying non-overt disseminated intravascular coagulation. Blood Coagul Fibrinolysis 2005, 16:69–74.PubMedView Article
- Franchini M, Manzato F: Update on the treatment of the disseminated intravascular coagulation. Hematology 2004, 9:81–85.PubMedView Article
- Levi M, de Jonge E, van der Poll T: New treatment strategies for disseminated intravascular coagulation based on current understanding of the pathophysiology. Ann Med 2004, 36:41–49.PubMedView Article
- Riewald M, Riess H: Treatment options for clinically recognized disseminated intravascular coagulation. Semin Thromb Haemost 1998, 24:53–59.View Article
- Levi M, de Jonge E, van der Poll T, ten Cate H: Novel approach to the management of disseminated intravascular coagulation. Crit Care Med 2000,28(Suppl):20–24.View Article
- Levi M, de Jonge E, van der Poll T: Therapeutic intervention in disseminated intravascular coagulation: have we made any progress in the last millennium? Blood Rev 2002, 16:29–34.View Article
- van der Poll T: Immunotherapy of sepsis. Lancet Infect Dis 2001, 1:165–174.PubMedView Article
- Levi M, de Jonge E, van der Poll T: Rationale for restoration of physiological anticoagulant pathways in patients with sepsis and disseminated intravascular coagulation. Crit Care Med 2001, 29:90–94.View Article
- du Toit H, Coetzee AR, Chalton DO: Heparin treatment in thrombin-induced disseminated intravascular coagulation in baboon. Crit Care Med 1991, 19:1195–1200.PubMedView Article
- Feinstein DI: Diagnosis and management of disseminated intravascular coagulation: the role of heparin therapy. Blood 1982, 60:284–287.PubMed
- Hoyle CF, Swisky DM, Freedman L, Hayhoe GFJ: Beneficial effect of heparin in the management of patients with APL. Br J Hematol 1988, 68:283–289.View Article
- Goldberg M, Ginsburg D, Mayer R, et al.: Is heparin necessary during induction chemotherapy for patients with acute promyelocytic leukemia? Blood 1987, 69:187–191.PubMed
- Rodeghiero F, Avvisati G, Castaman G, Barbui T, Mandelli F: Early deaths and anti-hemorrhagic treatments in acute promyelocytic leukemia. A GIMEMA retrospective study in 268 consecutive patients. Blood 1990, 75:2112–2117.PubMed
- Sakuragawa N, Hasegawa H, Maki M, Nakagawa M, Nakashima M: Clinical evaluation of low-molecular-weight heparin (FR-860) on disseminated intravascular coagulation (DIC) – a multicenter co-operative double blind trial in comparison with heparin. Thromb Haemost 1993, 72:475–500.
- Majumdar G: Idiopathic chronic DIC controlled with low-molecular-weight heparin. Blood Coagul Fibrinol 1996, 7:97–98.View Article
- Pernerstorfer T, Hollenstein U, Hansen JB: Heparin blunts endotoxin-induced coagulation activation. Circulation 1999, 100:2485–2490.PubMed
- Slofstra SH, van't Veer C, Buurman WA, Reitsma PH, ten Cate H, Spek CA: Low molecular weight heparin attenuates multiple organ failure in a murine model of disseminated intravascular coagulation. Crit Care Med 2005, 33:1365–1370.PubMedView Article
- Fujishima Y, Yokota K, Sukamoto T: The effect of danaparoid sodium (danaparoid) on endotoxin-induced experimental disseminated intravascular coagulation (DIC). Thromb Res 1998, 91:221–227.PubMedView Article
- Ontachi Y, Asakura H, Arahata M, et al.: Effect of combined therapy of danaparoid sodium and tranexamic acid on chronic disseminated intravascular coagulation associated with abdominal aortic aneurysm. Circ J 2004, 69:1150–1153.View Article
- Ibbotson T, Perry CM: Danaparoid. A review of its use in thromboembolic and coagulation disorders. Drugs 2002, 62:2283–2314.PubMedView Article
- Munoz MC, Montes R, Hermida J, Orbe J, Paramo JA, Rocha E: Effect of the administration of recombinant hirudin and/or tissue-plasminogen activator (T-PA) on endotoxin-induced disseminated intravascular coagulation model in rabbits. Br J Haematol 1999, 105:117–121.PubMedView Article
- Pernerstorfer T, Hollenstein U, Hansen JB, et al.: Lepirudin blunts endotoxin-induced coagulation activation. Blood 2000, 95:1729–1734.PubMed
- Schiffer ER, Reber G, De Moerloose P, Morel DR: Evaluation of unfractionated heparin and recombinant hirudin on survival in a sustained ovine endotoxin shock model. Crit Care Med 2002, 30:2689–2699.PubMedView Article
- Saito M, Asakura H, Jokaji H, et al.: Recombinant hirudin for the treatment of disseminated intravascular coagulation in patients with hematologic malignancy. Blood Coagul Fibrinolysis 1995, 6:60–64.PubMedView Article
- Bajaj MS, Bajaj SP: Tissue factor pathway inhibitor: potential therapeutic applications. Thromb Haemost 1997, 78:471–477.PubMed
- Abraham E: Tissue factor inhibition and clinical trials results of tissue factor pathway inhibitor in sepsis. Crit Care Med 2000, 28:S31–33.PubMedView Article
- Abraham E, Reinhart K, Svoboda P: Assessment of the safety of recombinant tissue factor pathway inhibitor in patients with severe sepsis: a multicenter, randomized, placebo-controlled, single-blind, dose escalation study. Crit Care Med 2001, 29:2081–2089.PubMedView Article
- Abraham E, Reinhart K, Demeyer I: Efficacy and safety of Tifacogin (recombinant tissue factor pathway inhibitor) in severe sepsis: a randomized controlled trial. JAMA 2003, 290:238–247.PubMedView Article
- Moons AH, Peters RJ, Cate H, et al.: Recombinant nematode anticoagulant protein c2, a novel inhibitor of tissue factor-factor VIIa activity, abrogates endotoxin-induced coagulation in chimpanzees. Thromb Haemost 2002, 88:627–631.PubMed
- De Jonge E, van der Poll T, Kesecioglu J, Levi M: Anticoagulant factor concentrates in disseminated intravascular coagulation: rationale for use and clinical experience. Semin Thromb Hemost 2001, 27:667–674.PubMedView Article
- Giudici D, Baudo F, Palareti G, Ravizza A, Ridolfi L, D'Angelo A: Antithrombin replacement in patients with sepsis and severe shock. Haematologica 1999, 84:452–460.PubMed
- Okajima K, Uchiba M: The anti-inflammatory properties of anti-thrombin III: new therapeutic implications. Semin Thromb Haemost 1998, 24:27–32.View Article
- Inthorn D, Hoffman J, Hartl W, Muhlbayer D, Jochum M: Effect of anti-thrombin III supplementation on inflammatory response in patients with severe sepsis. Shock 1998, 10:90–96.PubMedView Article
- Kessler CM, Tang Z, Jacobs HM, Szymanski LM: The suprapharmacological dosing of antithrombin concentrate for Staphylococcus aureus-induced disseminated intravascular coagulation in guinea pigs: substantial reduction in mortality and morbidity. Blood 1997, 89:4393–4401.PubMed
- Fourrier F, Jourdain M, Tournoys A: Clinical trial results with antithrombin III in sepsis. Crit Care Med 2000, 28:S38–43.PubMedView Article
- Eisele B, Lamy M, Thijs LG, et al.: Antithrombin III in patients with severe sepsis: a randomized placebo-controlled, double-blind multicenter trial plus meta-analysis on all randomized, placebo-controlled, double-blind trials with antithrombin III in severe sepsis. Intensive Care Med 1998, 24:663–672.PubMedView Article
- Baudo F, Caimi TM, de Cataldo F, et al.: Antithrombin III (ATIII) replacement therapy in patients with sepsis and/or post-surgical complications: a controlled, double blind, randomized multicenter study. Int Care Med 1998, 24:336–342.View Article
- Warren BL, Eid A, Singer P, KyberSept Trial Study Group, et al.: High-dose antithrombin III in severe sepsis: a randomized controlled trial. JAMA 2001, 286:1869–1878.PubMedView Article
- Kienast J, Juers M, Wiedermann CJ, for the KyberSept Investigators, et al.: Treatment effects of high-dose antithrombon without concomitant heparin in patients with severe sepsis with or without disseminated intravascular coagulation. J Thromb Haemost 2006, 4:90–97.PubMedView Article
- Gluck T, Opal SM: Advances in sepsis therapy. Drugs 2004, 64:837–859.PubMedView Article
- Fourrier F: Recombinant human activated protein C in the treatment of severe sepsis: an evidence-based review. Crit Care Med 2004, 32:S534–541.PubMedView Article
- Bernard GR, Hartman DL, Helterbrand JD, Fisher CJ: Recombinant human activated protein C (rhAPC) produces a trend toward improvement in morbidity and 28-day survival in patients with severe sepsis. Crit Care Med 1998, 27:S4.
- Bernard GR, Ely EW, Wright TJ, et al.: Safety and dose relationship of recombinant human activated protein C for coagulopathy in severe sepsis. Crit Care Med 2001, 29:2051–2059.PubMedView Article
- Bernard GR, Vincent JL, Laterre PF, Recombinant Human Protein C Worldwide Evaluation in Severe Sepsis (PROWESS) study group, et al.: Efficacy and safety of recombinant human activated protein C for severe sepsis. N Engl J Med 2001, 344:699–709.PubMedView Article
- Aoki N, Matsuda T, Saito H, CTC-111-IM Clinical Research Group, et al.: A comparative double-blind randomized trial of activated protein C and unfractionated heparin in the treatment of disseminated intravascular coagulation. Int J Hematol 2002, 75:540–547.PubMedView Article
- Dhainaut JF, Laterre PF, Janes JM, Recombinant Human Activated Protein C Worldwide Evaluation in Severe Sepsis (PROWESS) Study Group, et al.: Drotrecogin alfa (activated) in treatment of severe sepsis patients with multiorgan dysfunction: data from the PROWESS trial. Intensive Care Med 2003, 29:894–903.PubMed
- Vincent JL, Angus DC, Artigas A, Recombinant Human Activated Protein C Worldwide Evaluation in Severe Sepsis (PROWESS) Study Group, et al.: Effects of drotrecogin alfa (activated) on organ dysfunction in the PROWESS trial. Crit Care Med 2003, 31:834–840.PubMedView Article
- Vincent JL, Bernard GR, Beale R, et al.: Drotrecogin alfa (activated) treatment in severe sepsis from global open-label trial ENHANCE: further evidence for survival and safety and implications for early treatment. Crit Care Med 2005, 33:2266–2277.PubMedView Article
- Abraham E, Laterre P-F, Garg R, et al.: Drotrecogin alfa (activated) for adults with severe sepsis and a low risk of death. N Engl J Med 2005, 353:1332–1341.PubMedView Article
- Levi M, de Jonge E, van der Poll T: Recombinant human activated protein C (Xigris). Int J Clin Pract 2002, 56:542–545.PubMed
- Pastores SM: Drotrecogin alfa (activated): a novel therapeutic strategy for severe sepsis. Postgrad Med J 2003, 79:5–10.PubMedView ArticlePubMed Central
- Warren HS, Suffredini AF, Eichacker PQ, Munford RS: Risks and benefits of activated protein C treatment for severe sepsis. N Engl J Med 2002, 347:1027–1030.PubMedView Article
- Taylor FB, Chang A, Esmon CT, D'Angelo A, Vigano-D'Angelo S, Blick KE: Protein C prevents the coagulopathic and lethal effects of Escherichia coli infusion in the baboon. J Clin Invest 1987, 79:918–925.PubMedView ArticlePubMed Central
- Moscardo F, Perez F, de la Rubia J, et al.: Successful treatment of severe intra-abdominal bleeding associated with disseminated intravascular coagulation using recombinant activated factor VII. Br J Haematol 2001, 114:174–176.PubMedView Article
- Sallah S, Husain A, Nguyen NP: Recombinant activated factor VII in patients with cancer and hemorrhagic disseminated intravascular coagulation. Blood Coagul Fibrinolysis 2004, 15:577–582.PubMed
- Avvisati G, ten Cate JW, Buller HR, Mandelli F: Tranexamic acid for control of hemorrhage in acute promyelocytic leukemia. Lancet 1989, 2:122–124.PubMedView Article
- Norman KE, Cotter MJ, Stewart BJ: Combined anticoagulant and antiselectin treatments prevent lethal intravascular coagulation. Blood 2003, 101:921–928.PubMedView Article
- Pajkrt D, van der Poll T, Levi M, et al.: Interleukin-10 inhibits activation of coagulation and fibrinolysis during human endotoxemia. Blood 1997, 89:2701–2705.PubMed
- Reinhart K, Waheedullah K: Anti-tumor necrosis factor therapy in sepsis: update on clinical trials and lessons learned. Crit Care Med 2001, 29:S121–125.PubMedView Article
- Polderman KH, Girbes ARJ: Drug intervention trials in sepsis: divergent results. Lancet 2004, 363:1721–1723.PubMedView Article
- Cohen J, Carlet J: INTERSEPT: an international, multicenter, placebo-controlled trial of monoclonal antibody to human tumor necrosis factor-alpha in patients with sepsis. International Sepsis Trial Study Group. Crit Care Med 1996, 24:1431–1440.PubMedView Article
- Branger J, van den Blink B, Weijer S, et al.: Inhibition of coagulation, fibrinolysis, and endothelial cell activation by a p38 mitogen-activated protein kinase inhibitor during human endotoxemia. Blood 2003, 101:4446–4448.PubMedView Article
- Verbon A, Meijers JC, Spek CA: Effects of IC14, an anti-CD14 antibody, on coagulation and fibrinolysis during low-grade endotoxemia in humans. J Infect Dis 2003, 187:55–61.PubMedView Article
- Ziegler EJ, Fisher CJ Jr, Sprung CL, et al.: Treatment of gram-negative bacteremia and septic shock with HA-1A human monoclonal antibody against endotoxin. A randomized, double-blind, placebo-controlled trial. The HA-1A Sepsis Study Group. N Engl J Med 1991, 324:429–436.PubMedView Article
- McCloskey RV, Straube RC, Sanders C, Smith SM, Smith CR: Treatment of septic shock with human monoclonal antibody HA-1A. A randomized, double-blind, placebo-controlled trial. CHESS Trial Study Group. Ann Intern Med 1994, 121:1–5.PubMed
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