Figure 3

(A) Effects of progressive 5' deletions of the TF gene on basal and bFGF inducible TF transcriptional activity. For determination of promoter activity raw data were tabulated as the ratio of firefly luciferase activity to that of Renilla luciferase activity (means ± SD, n = 18), and values were reported as relative to the value for the control plasmid (assigned a value of 100). *p < 0.05 and **p < 0.01. Constructs of transfected plasmids are indicated at the left panel. TATA: TATA box. (B) Effects of point mutations in NF-κB like site and SP-1/Egr-1 like site on TF promoter activity (n = 3). Promoter activity was determined and values were reported as relative to the value for the TF full without bFGF stimulation. Basal expression of luciferase activity of TF promoter vector was detected by the TF Full luciferase vector, which has normal NF-κB like site (5'-CGGAGTTTCC-3'). One mutation vector has two point mutations in NF-κB like site as underlined (5'-CGGAGTTTGG-3'). Another mutation vector has two point mutations in NF-κB like site as underlined (5'-CGGAGTTTGG-3') and one point mutation in SP-1/Egr-1 like site (5'-AGGCA GGGCAGGGGTG-3'). *p < 0.05 and **p < 0.01.