Figure 1

A), Examples of thrombotic profiles generated by the perfusion of untreated FXa-anticoagulated samples of blood from a healthy individual on fibrillar collagen at different shear rates (representative of 5 different donors). The profiles lose their linearity as the wall shear rate increases. At least three main factors may contribute to the exponential curve observed for wall shear rates > 500/sec. i) an increase amount of platelets in the vicinity of the collagen surface, ii) a participation of von Willebrand factor in the thrombotic deposits for higher wall shear rate conditions, iii) an exponential increase in shear rate at the apex of the thrombi. As a result, it is expected that exponential profiles could be also observed at lower wall shear rates but for longer perfusion period. B), Representative thrombotic profiles of untreated blood perfused at 1000/sec, 15, 30, 45, 60 75 and 90 minutes post blood collection from a single individual. The working range of the assay post blood collection is plotted as mean + S.E.M for Peak thrombus size in C and slope before peak in D.