Figure 3
Effect of SMTP-7 on plasma level of Pm-AP in normal mice. Mice were intravenously injected with SMTP-7 at a bolus dose of 5 mg kg-1 (n = 5) or 10 mg kg-1 (n = 5). After 60 min, blood was drawn to determine Pm-AP levels in plasma by fibrinogen zymography. Control animals (n = 5) received saline. (A) Representative zymogram. Plasmas from 5 animals in each group were combined and aliquots (2 μl) of the mixtures were subjected to zymography. Human Pm-AP (hPm-AP, 1.7 ng) was resolved as the standard. (B) The summary of the quantification of Pm-AP. Plasma (2 μl) from each of the five mice was independently subjected to zymographic analysis. Data were calibrated by comparing the intensity of each lysis band with that of the standard human Pm-AP. The mean + SD of the data obtained from each animal is shown. *, P < 0.05 and **, P < 0.01 compared with control by the Dunnett's multiple comparison test. (C) Authenticity confirmation. The mixture of plasma in the 5 mg kg-1 SMTP-7 group was treated with anti-plasminogen IgG (Anti-Plg), anti-α2-antiplasmin IgG (Anti-AP), or buffer before the zymographic determination of Pm-AP.