Figure 1
From: Correction of microplate location effects improves performance of the thrombin generation test
Row artifacts (location effects) in the TGT assay for procoagulant activity of immune globulin products. A. 96 well microplate diagram showing location of row standards (shaded circles) included in each TGT experiment. B. Effect of row location on the thrombin generation curve in a typical experiment. C. Effect of row location on thrombin peak height (TPH) under a variety of conditions. TPHs were normalized on the first recalcified row (either row A or H). Means ± S.D. are shown. Black bars: n = 19 assays; FXI-deficient plasma (FXIDP) mixed with IVIG sample #1. Order of recalcification: row A ➔ row H. Dark gray bars: n = 2; conditions as in black bars but the order of row recalcification is reversed (row H ➔ row A). Light gray bars: n = 7; conditions as in black bars but IVIG sample #2 (higher activity) was used. White bars: n = 7; conditions as in light gray bars but normal pooled plasma was used. Symbols above bars indicate statistically significant difference versus first recalcified row using paired t-test (P < 0.05).