Figure 2
From: Correction of microplate location effects improves performance of the thrombin generation test
Random and systemic location effects observed for serially diluted procoagulant IVIG product. A. 96 well microplate diagram showing row-to-row location of serially diluted IVIG sample. Blue and red colors on this and other panels denote 1.6- and 16.8-fold dilutions, respectively. B. Thrombin generation profiles in FXIDP mixed with serially diluted thrombogenic lot of IVIG. Data from row B. C. Effect of row location on the TPH measured in the TGT assay. Note random row variations, e.g., relatively low thrombin peaks on rows B and F, and systemic trend to TPH reduction on rows A âž” B. D. Linear fit was used to demonstrate that row-to-row drift is dependent on the activity of the sample and time between row recalcification and placement into the reader (outside time).