Figure 5
From: Correction of microplate location effects improves performance of the thrombin generation test
Location effects in the commercial thrombin generation assay (CAT). A. Symmetrical plate layout design in which FXIa titration was repeated on columns 1 through 6, and, in reversed direction of sample dilutions, on columns 7 through 12. Colored circles denote two algorithms of duplicate well averaging: orange circles demonstrate symmetrically positioned duplicate wells; blue circles demonstrated adjacent position of duplicate wells. “TC” denotes thrombin calibrator wells. B. Adjacent averaging (blue lines) reveals column drifts in TPH while symmetrical averaging of duplicate samples corrects this trend (orange lines). Means ± S.D. (n = 2 wells). C. Effect of column location on the FXIa dose - TPH response curves in the case of adjacent averaging. D. Symmetrically averaged columns overlap indicating corrected location artifacts. Means ± S.D. (n = 2 wells).