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Figure 4 | Thrombosis Journal

Figure 4

From: Purification and characterization of mutant miniPlasmin for thrombolytic therapy

Figure 4

Purification of mutant mPlg using Superdex-200 SEC column. Results of wild type and all 16 mutants are shown. Each mutant was expressed, refolded, and purified as described in the methods section. Non-reduced SDS-PAGE (4-12% polyacrylamide gradient gel) was used to access the folding. Each batch of refolding was performed with the wild-type enzyme as a positive control. On the SDS-PAGE, the lower major band in the second peak is the folded enzyme. In the SEC graph, the folded, active peak is indicated with red arrows.

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