VacA induces CD62P expression on platelets. PRP were incubated with 120nM VacA or H-VacA. After 30 minutes, PRP were staining with anti-CD62PE or mouse control IgG1-PE for 20 minutes in the dark. Platelets were mixed with 1% paraformaldehyde in PBS, and samples were analyzed with a flow cytometer. The data expressed as per cent increase over the control are compiled from 6 experiments.