Figure 1

Characterization of Pxn-KD platelets. Bone marrow cells transduced with LentiLox-sh-control-GPIbα (Control) or LentiLox-sh-paxillin-GPIbα (Pxn-KD) at an MOI of 5 were transplanted into lethally irradiated recipient mice. (A) The numbers of GFP-positive cells (%) among white blood cells (WBCs), red blood cells (RBCs), and platelets (Plts) in peripheral blood at 30 days after transplantation were assessed by flow cytometry. Columns and error bars represent the mean ± s.d. (n = 10). (B) Absolute number of GFP-positive platelets in peripheral blood at 30 days after transplantation. Columns and error bars represent the mean ± s.d. (n = 10). (C) Platelet lysates were immunoblotted with the indicated antibodies. Data are for two mice with >80% of platelets expressing GFP in each experiment. (D) The morphology of control and Pxn-KD platelets was examined by transmission electron microscopy. Bars = 2 μm. (E) Platelet length (left panel) and area (right panel) were quantified by ImageJ software. Columns and error bars represent the mean ± s.d. (n = 53–72). Statistical significance was determined by the Student’s t-test. *P < 0.05 and ***P < 0.001 vs. control.