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Figure 3 | Thrombosis Journal

Figure 3

From: Paxillin is an intrinsic negative regulator of platelet activation in mice

Figure 3

The release reaction is enhanced in Pxn-KD platelets. Bone marrow cells transduced with LentiLox-sh-control-GPIbα (Control) or LentiLox-sh-paxillin-GPIbα (Pxn-KD) at an MOI of 5 were transplanted into lethally irradiated recipient mice. (A) P-selectin expression on GFP-positive platelets stimulated with or without 1 mmol/L AYPGKF or 50 ng/mL convulxin was assessed by flow cytometry. The plots represent the degree of GFP expression (horizontal) and P-selectin expression (vertical). (B) Columns and error bars represent the mean ± s.d. of P-selectin expression after stimulation in GFP-positive platelets (n = 3–5). (C–E) Washed platelets were stimulated with the indicated agonist for 15 min, and then the concentrations of PF4 (C), serotonin (D), and TxB2(E) were measured in the supernatants. Columns and error bars represent the mean ± s.d. (n = 5). Open bars: control platelets; black bars: Pxn-KD platelets. Statistical significance was determined using Student’s t-test. *P < 0.05, **P < 0.01, and ***P < 0.001 vs. control.

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