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Figure 1 | Thrombosis Journal

Figure 1

From: Analysis of blood coagulation in mice: pre-analytical conditions and evaluation of a home-made assay for thrombin-antithrombin complexes

Figure 1

Antithrombin (lane AT), thrombin (lane T) and in vitro TAT complexes (lane TAT) were separated on a 7.5% acrylamide gel and transferred to a nitrocellulose filter. Plasma samples of control mice (lane 1) or mice treated with 50 μg/ml LPS for 2 (lane 2), 6 (lane 3) and 24 hours (lane 4) were separated also. The filters were incubated with 1000-fold diluted serum from a rabbit immunized with thrombin (A), or serum from a rabbit immunized with AT (B).

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