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Figure 2 | Thrombosis Journal

Figure 2

From: Analysis of blood coagulation in mice: pre-analytical conditions and evaluation of a home-made assay for thrombin-antithrombin complexes

Figure 2

Selection of capture and conjugating antibody. 96-wells plates were coated with either rabbit anti-mouse thrombin antibodies (Panel A) or rabbit anti-rat antithrombin antibodies (Panel B) prior to incubation with two-fold serial dilutions of in vitro TAT-complexes. Plates coated with rabbit α-AT antibodies were subsequently incubated with serial dilutions of DIG-conjugated rabbit α-thrombin antibodies, whereas plates coated with rabbit α-thrombin antibodies were incubated with serial dilutions of DIG-conjugated rabbit α-AT antibodies.

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