Skip to main content

Advertisement

Table 1 Comparison of sampling techniques for different coagulation parameters: Vena cava puncture (VCP) vs tail cut (TC) as measured by Prothrombin Time (PT) and activated Partial Thromboplastin Time (aPTT) in plasma of untreated negative control mice

From: Systemic coagulation parameters in mice after treatment with vascular targeting agents

Assay: PT mean (SD) aPTT mean (SD) number of samples not evaluable number of samples analyzed % of samples not evaluable
Sampling technique: [s] [% of standard]* [s] [n] [n] [%]
VCP 1:5 9.2 (1.0) 94 (10) 31.7 (4.6) 3 15 20%
VCP 1:10 7.8 (0.1) 116 (13) 23.2 (0.8) 0 10 0%
TC 1:5 - - - 3 3 100 %
TC 1:10 - - - 4 4 100 %
Ref.values: 8–10 80–120 32–36/23–25†    
  1. * where the clotting time of a normal plasma standard was defined as 100%
  2. † normal values depended on citrate:blood ratio, and were 32–36 s for 1:5 ratio and 23–25 s for 1:10 ratio