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Table 3 Correlation of results of three different assays determining thrombin activity in plasma of mice treated with lipopolysaccharides (LPS) in the presence and absence of the thrombin inhibitor hirudin

From: Systemic coagulation parameters in mice after treatment with vascular targeting agents

   Soluble Fibrin determined by agglutination assay Soluble Fibrin determined by chromogenic assay Thrombin-Antithrombin complexes
Treatment of mice sample no. semiquantitative result* qualitative result (fold increase over negative control) qualitative result [ng/ml]
LPS, 15 min 1 - - (1.1) - 3
  2 - - (1.3) - 0
  3 - - (1.1) - 2
  4 (+) + (2.2) - 3
  5 ++ + (3.7) - 8
LPS, 2 h 6 (+) - (1.2) - 6
  7 + + (2.2) + 21
  8 + + (2.2) + 34
  9 ++ + (2.4) + 20
  10 ++ + (2.1) + 22
LPS + hirudin, 2 h 11 - + (2.2) - 10
  12 - - (1.6) - 10
  13 - - (1.5) - 10
  14 (+) - (1.8) - 5
  15 (+) - (1.8) - 5
no treatment 16 - - (1.5) - 2
  17 - - (1.1) - 0
  18 - - (0.9) - 0
  1. * where ++ corresponds to 0.05 thrombin units/ml, + corresponds to 0.025 thrombin units/ml, (+) stands for borderline result (less than +, but not conclusively -) and – stands for negative (see also Figure 1C).