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Table 3 Correlation of results of three different assays determining thrombin activity in plasma of mice treated with lipopolysaccharides (LPS) in the presence and absence of the thrombin inhibitor hirudin

From: Systemic coagulation parameters in mice after treatment with vascular targeting agents

  

Soluble Fibrin determined by agglutination assay

Soluble Fibrin determined by chromogenic assay

Thrombin-Antithrombin complexes

Treatment of mice

sample no.

semiquantitative result*

qualitative result (fold increase over negative control)

qualitative result

[ng/ml]

LPS, 15 min

1

-

- (1.1)

-

3

 

2

-

- (1.3)

-

0

 

3

-

- (1.1)

-

2

 

4

(+)

+ (2.2)

-

3

 

5

++

+ (3.7)

-

8

LPS, 2 h

6

(+)

- (1.2)

-

6

 

7

+

+ (2.2)

+

21

 

8

+

+ (2.2)

+

34

 

9

++

+ (2.4)

+

20

 

10

++

+ (2.1)

+

22

LPS + hirudin, 2 h

11

-

+ (2.2)

-

10

 

12

-

- (1.6)

-

10

 

13

-

- (1.5)

-

10

 

14

(+)

- (1.8)

-

5

 

15

(+)

- (1.8)

-

5

no treatment

16

-

- (1.5)

-

2

 

17

-

- (1.1)

-

0

 

18

-

- (0.9)

-

0

  1. * where ++ corresponds to 0.05 thrombin units/ml, + corresponds to 0.025 thrombin units/ml, (+) stands for borderline result (less than +, but not conclusively -) and – stands for negative (see also Figure 1C).