Figure 4
Effect of perfusion flow rate on monocyte adherence to tumor cells. Monocytes (1 × 106/ml) were perfused across a monolayer of A375 cells attached to a coverslip in a perfusion stage incubator for 1 h at 37°C, and non-adherent cells were washed off by perfusion to waste for 10 min. Monocyte adherence was maximal at a flow rate of 0.5 ml/min, and linearly decreased as the flow rate was increased to 1, 1.5, and 2 ml/min.