Figure 5
Tumor cells were grown on 40 mm coverslips to confluence and labeled with Calcein AM (green). Monocytes were labeled with DiI (C18; red), made to 1 × 106 cells/ml and continuously perfused across the tumor cells at 0.5 ml/min for 1 h. (Left) monocyte adherence to untreated A375 cells. (Right) monocyte (pre-treated with sFn) adherence to sFn pre-treated A375 cells. sFn pre-treatment of both effector and target cells inhibited monocyte adherence.