Figure 8
Effect of specific blocking peptides designated P3 (binds CD54 binding site on sFn) and P4 (binds to αMβ2 binding site on sFn) on sFn inhibition of monocyte/tumor cell adherence under flow conditions. (from left to right): sFn treatment of monocytes and A375 cells (n = 25) significantly (P < 0.01 compared to untreated control; n = 10) inhibited cell adherence. Pretreatment of sFn with P3 and P4 restored cell adherence to levels not significantly different to the untreated control (P < 0.05 to fibrin; n = 5; P > 0.05 to control). Pre-treatment of tumor cells and/or monocytes with either P3+P4, P3 alone or P4 alone were not inhibitory (P > 0.05; n = 5 in each case, compared to untreated control).