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Table 3 Russel Viper Venom Times in normal plasma. RVVT were measured in NP with two different phospholipid reagents, with crude cephalin diluted 1/200 or 1/10 000, respectively. Addition of unfractionated heparin to plasma without polybrene gave false positive Lupus Ratios, while addition of heparin to plasma with polybrene gave correct result. Results are given as means of three runs. RVVT-based Lupus Ratio <1.11 = negative result.

From: Detection and quantification of lupus anticoagulants in plasma from heparin treated patients, using addition of polybrene

Heparin concentration Polybrene concentration Mean clotting time with cephalin 1/200 Mean clotting time with cephalin 1/10 000 Lupus Ratio
0.0 U/ml 0.00 μg/ml 29.8 seconds 46.3 seconds ------
0.0 U/ml 3.96 μg/ml 30.4 seconds 48.8 seconds 1.03 (neg.)
0.5 U/ml 3.96 μg/ml 29.8 seconds 46.9 seconds 1.01 (neg.)
0.5 U/ml 0.00 μg/ml 38.1 seconds 75.4 seconds 1.27 (false pos.)
  1. Russel Viper Venom Times were measured with two different reagents, with crude cephalin diluted 1/200 or 1/3200, respectively. Addition of unfractionated heparin to plasma without polybrene gave false positive Lupus Ratios, while addition of heparin to plasma with polybrene gave correct result. RVVT-based Lupus Ratio ≤ 1.10 = negative.