Table 3 Russel Viper Venom Times in normal plasma. RVVT were measured in NP with two different phospholipid reagents, with crude cephalin diluted 1/200 or 1/10 000, respectively. Addition of unfractionated heparin to plasma without polybrene gave false positive Lupus Ratios, while addition of heparin to plasma with polybrene gave correct result. Results are given as means of three runs. RVVT-based Lupus Ratio <1.11 = negative result.
Heparin concentration | Polybrene concentration | Mean clotting time with cephalin 1/200 | Mean clotting time with cephalin 1/10 000 | Lupus Ratio |
|---|---|---|---|---|
0.0 U/ml | 0.00 μg/ml | 29.8 seconds | 46.3 seconds | ------ |
0.0 U/ml | 3.96 μg/ml | 30.4 seconds | 48.8 seconds | 1.03 (neg.) |
0.5 U/ml | 3.96 μg/ml | 29.8 seconds | 46.9 seconds | 1.01 (neg.) |
0.5 U/ml | 0.00 μg/ml | 38.1 seconds | 75.4 seconds | 1.27 (false pos.) |