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Table 5 Effect of pair of agonists compared with single agonist in thrombin generationparameters (n = 30) using low doses agonists and rTF. Final concentration of agonists: ADP 0.2 μM; sodium arachidonate (AA) 0.125 mmol/L; collagen (Col) 0.05 μg/mL, recombinant tissue factor (rTF) 2.5 pmol/L

From: Thrombin generation by activated factor VII on platelet activated by different agonists. Extending the cell-based model of hemostasis

  B C D E
  Lag Time (min.) mean ± SD Time to Peak (min.) mean ± SD PTG (nmol/L) mean ± SD AUC (nmol/L) mean ± SD
1 ADP 15.4 ± 4.0 23.2 ± 4.9 155 ± 41 1260 ± 474
2. AA +ADP 11.3 ± 2.5 17.3 ± 2.6 198 ± 45 1604 ± 188
3. Col 14.3 ± 3.3 21.6 ± 3.7 148 ± 44 1392 ± 264
4. AA+Col 11.8 ± 2.5 17.8 ± 3.3 187 ± 53 1551 ± 257
5. AA 12.9 ± 3.1 19.0 ± 3.4 179 ± 58 1488 ± 286
6. rTF 5.8 ± 1.8 12.9 ± 2.4 154 ± 50 1495 ± 337
  1. Parameters of TG in PRP from 30 normal volunteers. PTG: Peak to thrombin generation. AUC: Area under the curve. AA, ADP or Col were compared with their combining effects. Also rTF, 2.5 pM was compared with the other agonists.
  2. Lag Time: 1 vs 2 <0.001; 3 vs 4 <0.01; 2 vs 5 = NS; 4 vs 5 = NS 6 vs 1 to 5 <0.001
  3. Time to Peak 1 vs 2 <0.001; 3 vs 4 <0.001; 2 vs 5 = NS; 4 vs 5 = NS 6 vs 1 to 5 <0.001
  4. PTG 1 vs 2 <0.001; 3 vs 4 <0.01; 2 vs 5 =NS; 4 vs 5 =NS 6 vs 1 to 5 =NS
  5. AUCo→35min 1 vs 2 <0.001; 3 vs 4 =NS; 2 vs 5 =NS; 4 vs 5 =NS 6 vs 1 to 5 =NS
  6. rTF appears as more effective than each agonist alone or their combination in shorten times parameters of TG, but effects are not different when amount parameters are considered.