Figure 2

In vitro anticoagulant response in rat plasma to added APC variants. Different concentrations of WT hAPC, or the two mutants of hAPC, with or without recombinant hPS (10 μg/ml) were added in coagulation tests using either the APTT reagent Platelin LS (A) or the tissue-factor reagent Simplastin Excel (B). Note that the APTT reagent was diluted 1/2 to make the assay more sensitive. Therefore, it can be concluded that the hAPC variants + hPS have lower anticoagulant potential than the bAPC+bPS combination shown in figure 1, even though the prolongation of clotting times are similar. Each experiment was performed three times with measurements in duplicate. Mean values are presented. The indicated concentrations of hAPC represent the final concentrations. The experiments without hPS were performed and presented in our previous study [25] and the same results are presented here again in conjunction with the tests with hPS, to make comparisons possible.