Figure 3

Inactivation of APC in rat plasma. The different APC variants were added to rat plasma and at various time points; the remaining amidolytic activity was measured at 405 nm using the synthetic substrate S-2366. The absorbance reading of plain rat plasma was subtracted from the mean of two absorbance readings at each time point and the difference was used for presentation. The three recombinant hAPC variants were tested in one experiment and bAPC was tested in another experiment and the results were combined into one figure.