Figure 2
From: Plasma TF activity predicts cardiovascular mortality in patients with acute myocardial infarction
Differential analysis of soluble TF (top row) and microparticle-associated TF (bottom row) in human plasma. In both supernatant and pellet fractions, the frequencies of TF+Annexin+ microparticles were analyzed by FACS analysis. Representative dot blots of a patient with ACS are shown with (A, F) light-scatter-staining, and (B, G) specific-staining. (C, H) Concentrations of TF antigen were measured by immunoassay. (D, I) Plasma TF activity with or without (+/-) anti-TFPI-1 was measured by FXa generation assay. (E, J) Specific microparticle-associated TF activity was measured following antibody capture of microparticles. Values are expressed as mean ± SEM.