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Figure 4 | Thrombosis Journal

Figure 4

From: Enhancement of fibrinogen-triggered pro-coagulant activation of monocytes in vitro by matrix metalloproteinase-9

Figure 4

Inhibitors of the p38 MAPK and MEK1/2 pathway block fibrinogen-triggered pro-coagulant activation of human monocytes. As integrins primarly signal via p38 MAPK and MEK1/2 pathways, experiments with inhibitors against p38 MAPK, MEK 1/2 and JNKII were performed. Pre-exposure of the monocytes to the p38 MAPK inhibitor SB 202190 and the MEK 1.2 inhibitor U0126 led to a significant increase in coagulation time whereas blocking JNKII with its inhibitor had no effect on coagulation. Activation of NFκB by certain stimuli switches monocytes from their resting state into activated monocytes that transform into macrophages. Blocking NFκB with MG-132 inhibited the activation of monocytes by fibrinogen, the pro-coagulant activity of MG-132-treated cells was 107% of un-treated control cells. Results are given as mean ± SEM, n = 3.*: p < 0.05.

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