|
Parameter
|
Time
|
Sprague-Dawley
|
Copenhagen
|
|---|
| | |
PE
|
Sham
|
Sig.
|
PE
|
Sham
|
Sig
|
|---|
|
Weight (g)
|
24 hr
|
399 ± 5
|
392 ± 4
|
n.s.
|
240 ± 8
|
254 ± 2
|
n.s.
|
| |
5 day
|
402 ± 5
|
393 ± 5
|
n.s.
|
244 ± 8
|
245 ± 4
|
n.s.
|
|
Pleural WBC (×105)
|
24 hr
|
108 ± 19
|
109 ± 32
|
n.s.
|
27 ± 4
|
32 ± 4
|
n.s.
|
| |
5 day
|
107 ± 16
|
109 ± 4
|
n.s.
|
39 ± 8
|
31 ± 3
|
n.s.
|
|
BAL WBC (×103)
|
24 hr
|
543 ± 45
|
337 ± 35
|
*
|
491 ± 26
|
415 ± 51
|
n.s.
|
| |
5 day
|
587 ± 82
|
514 ± 40
|
n.s.
|
449 ± 65
|
435 ± 44
|
n.s.
|
|
BAL Chemotaxis (×103)
|
24 hr
|
52 ± 9
|
33 ± 3
|
n.s.
|
16 ± 1
|
17 ± 2
|
n.s.
|
| |
5 day
|
41 ± 4
|
37 ± 4
|
n.s.
|
18 ± 2
|
13 ± 5
|
n.s.
|
|
BAL MPO (U/mL)
|
24 hr
|
229 ± 79
|
104 ± 23
|
n.s.
|
128 ± 11
|
152 ± 57
|
n.s.
|
| |
5 day
|
256 ± 133
|
301 ± 65
|
n.s.
|
142 ± 87
|
102 ± 19
|
n.s.
|
|
BAL MCP-1 (pg/mL)
|
24 hr
|
137 ± 90
|
42 ± 14
|
n.s.
|
188 ± 112
|
134 ± 48
|
n.s.
|
| |
5 day
|
24 ± 13
|
101 ± 80
|
n.s.
|
187 ± 105
|
228 ± 59
|
n.s.
|
|
BAL CINC-1 (pg/mL)
|
24 hr
|
62 ± 32
|
32 ± 11
|
n.s.
|
66 ± 14
|
38 ± 12
|
n.s.
|
| |
5 day
|
21 ± 6
|
30 ± 12
|
n.s.
|
36 ± 8
|
41 ± 7
|
n.s.
|
|
BAL CINC-2 (pg/mL)
|
24 hr
|
638 ± 51
|
554 ± 32
|
n.s.
|
267 ± 23
|
220 ± 28
|
n.s.
|
| |
5 day
|
610 ± 45
|
552 ± 40
|
n.s.
|
266 ± 42
|
280 ± 18
|
n.s.
|
|
BAL CINC-3 (pg/mL)
|
24 hr
|
21 ± 1
|
21 ± 0.4
|
n.s.
|
19 ± 4
|
19 ± 8
|
n.s.
|
| |
5 day
|
20 ± 0.3
|
20 ± 0.1
|
n.s.
|
18 ± 6
|
15 ± 4
|
n.s.
|
- Values are mean ± standard error, where n = 10 Sprague-Dawley, n = 4-5 Copenhagen. *Indicates a value significantly different from vehicle-treated Sham animals (Bonferroni-adjusted p < 0.05, t-Test). Abbreviations: not significant (n.s.); pleural white blood cell count (WBC); bronchoalveolar lavage fluid (BAL); white blood cell count (WBC); in vitro neutrophil chemotaxis in response to BAL fluid (Chemotaxis) myeloperoxidase activity (MPO); monocyte chemotactic factor (MCP); cytokine-induced neutrophil chemotactic factor (CINC).
