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Table 1 FV Activity in NHP and in 9 Patients That Developed Disseminated Intravascular Coagulation (DIC)

From: Development of a microplate coagulation assay for Factor V in human plasma

Sample 1-stage assay Activity (Units/ml) 1-stage assay Extent (Units) 1-stage assay Initial Rate (mUnits/min) 2-stage assay Activity (Units/ml) 2-stage assay Extent (Units) 2-stage assay Initial Rate (mUnits/min) Total activity (Units/ml)
NHP 1.02 0.363 744.96 7.93 0.433 375.84 6.91
Patient 1 0.36 0.404 583.80 3.84 0.432 249.96 3.48
Patient 2 0.67 0.416 704.04 5.28 0.469 323.16 4.61
Patient 3 0.31 0.435 562.08 3.92 0.453 294.96 3.61
Patient 4 0.39 0.435 617.04 4.14 0.462 372.60 3.75
Patient 5 0.73 0.401 641.40 5.91 0.433 354.24 5.18
Patient 6 0.45 0.403 600.72 4.16 0.445 393.96 3.71
Patient 7 0.49 0.395 575.40 4.89 0.449 357.48 4.40
Patient 8 0.19 0.448 489.00 2.89 0.450 330.48 2.70
Patient 9 0.64 0.423 699.48 5.11 0.455 417.24 4.47
  1. The FV 1-stage, 2-stage, and total activity in normal pooled human reference plasma (NHP) and the 9 DIC patient plasma samples were determined from the FV 1-stage microplate assay standard curve of time of clot formation vs FV activity (Figure 2A) and are given in Units/ml. The initial rates (Initial rate of increase in A405nm over first five time points in mUnits/min) and extents (Maximum A405nm - Minimum A405nm) of clot formation in the FV 1- and 2-stage microplate assay are also shown.