Development of a microplate coagulation assay for Factor V in human plasma

Thrombosis Journal

Table 1 FV Activity in NHP and in 9 Patients That Developed Disseminated Intravascular Coagulation (DIC)

Sample	1-stage assay Activity (Units/ml)	1-stage assay Extent (Units)	1-stage assay Initial Rate (mUnits/min)	2-stage assay Activity (Units/ml)	2-stage assay Extent (Units)	2-stage assay Initial Rate (mUnits/min)	Total activity (Units/ml)
NHP	1.02	0.363	744.96	7.93	0.433	375.84	6.91
Patient 1	0.36	0.404	583.80	3.84	0.432	249.96	3.48
Patient 2	0.67	0.416	704.04	5.28	0.469	323.16	4.61
Patient 3	0.31	0.435	562.08	3.92	0.453	294.96	3.61
Patient 4	0.39	0.435	617.04	4.14	0.462	372.60	3.75
Patient 5	0.73	0.401	641.40	5.91	0.433	354.24	5.18
Patient 6	0.45	0.403	600.72	4.16	0.445	393.96	3.71
Patient 7	0.49	0.395	575.40	4.89	0.449	357.48	4.40
Patient 8	0.19	0.448	489.00	2.89	0.450	330.48	2.70
Patient 9	0.64	0.423	699.48	5.11	0.455	417.24	4.47

The FV 1-stage, 2-stage, and total activity in normal pooled human reference plasma (NHP) and the 9 DIC patient plasma samples were determined from the FV 1-stage microplate assay standard curve of time of clot formation vs FV activity (Figure 2A) and are given in Units/ml. The initial rates (Initial rate of increase in A405nm over first five time points in mUnits/min) and extents (Maximum A405nm - Minimum A405nm) of clot formation in the FV 1- and 2-stage microplate assay are also shown.

ISSN: 1477-9560