Emilin2 mRNA Expression in Tissues and Mouse Cells. GAPDH from the same cDNA samples used as endogenous control. A. Expression of Emilin2 in lung, heart, aorta and bone marrow cells was determined by real-time PCR. Aorta, n = 3; others, n = 5. The mRNA levels are presented as fold change relative to lung. The values are mean ± SEM n = 3-5. B. Expression of Emilin2 in mouse cell lines. The mRNA levels are presented as fold change relative to EOMA. EOMA, mouse endothelial cell line; NIH3T3, mouse fibroblast cell line; Raw264.7, mouse macrophage-like cell line. The values are mean ± SEM of 3 independent experiments. Statistical differences compared to EOMA were determined by ANOVA and Newman-Kuels post-test. **P < 0.01, ***P < 0.001.