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Fig. 1 | Thrombosis Journal

Fig. 1

From: Analysis of the potential of cancer cell lines to release tissue factor-containing microvesicles: correlation with tissue factor and PAR2 expression

Fig. 1

Quantitative analysis of TF mRNA, protein and surface activity of cell lines. a Total RNA was isolated from each cell lines (5 × 105 cells) and 100 ng used to determine the absolute amount of TF mRNA in each sample using a quantitative real-time RT-PCR. (n = 3) b Cells (5 × 105) were also lysed and TF protein quantified using a TF-antigen EIA kit. (n = 3) C) Cell surface (5 × 104) TF activities were measured for each cell lines using a modified chromogenic thrombin-generation assay (n = 3)

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