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Fig. 3 | Thrombosis Journal

Fig. 3

From: Analysis of the potential of cancer cell lines to release tissue factor-containing microvesicles: correlation with tissue factor and PAR2 expression

Fig. 3

Time course of the microvesicle-associated TF activity released into the media by the cell lines. Cells (2 × 105) were seeded out in 6-well plates and pre-adapted to respective serum-free medium. Microvesicle release was induced by incubation with PAR2-AP; SLIGRL; (20 μM). The released cell-derived microvesicles were isolated by ultracentrifugation at intervals up to 90 min and resuspended in Tris-saline. Microvesicle-associated TF activity was measured using the thrombin-generation assay, for each sample (n = 3)

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Thrombosis Journal

ISSN: 1477-9560

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