Fig. 4
Analysis of minimal and maximal levels of released microvesicle-associated TF activity by the cell lines. Cells (2 × 105) were seeded out in 6-well plates and pre-adapted to respective serum-free medium. Microvesicle release was induced by incubation with PAR2-AP; SLIGRL; (20 μM). The released cell-derived microvesicles were then isolated by ultracentrifugation prior to activation (resting), and at the interval with the maximum level of microvesicle-associated TF activity (see Fig. 3). The microvesicles were resuspended in Tris-saline and TF activity in each sample was measured using the thrombin-generation assay, in each sample. (n = 3; * = p < 0.05 vs. the respective non-activated sample) (N/T = Not tested)