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Fig. 5 | Thrombosis Journal

Fig. 5

From: Analysis of the potential of cancer cell lines to release tissue factor-containing microvesicles: correlation with tissue factor and PAR2 expression

Fig. 5

Analysis of minimal and maximal levels of released microvesicles by the cell lines. Cells (2 × 105) were seeded out in 6-well plates and pre-adapted to respective serum-free medium. Microvesicle release was induced by incubation with PAR2-AP; SLIGRL; (20 μM) for the durations determined in Fig. 3. The released cell-derived microvesicles were then isolated by ultracentrifugation at the interval with the maximum level of microvesicle-associated TF activity (see Fig. 3). Samples of cells were also incubated for the same periods but without activation (resting) and microvesicles isolated. The microvesicles were resuspended in Tris-saline and the density in each sample determined using the Zymuphen MP assay kit. (n = 3; * = p < 0.05 vs. the respective non-activated sample)

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