Analysis of the potential of cancer cell lines to release tissue factor-containing microvesicles: correlation with tissue factor and PAR2 expression

Ettelaie, Camille; Collier, Mary EW; Featherby, Sophie; Benelhaj, Naima E.; Greenman, John; Maraveyas, Anthony

doi:10.1186/s12959-016-0075-3

Table 1 Correlations between cell line parameters

From: Analysis of the potential of cancer cell lines to release tissue factor-containing microvesicles: correlation with tissue factor and PAR2 expression

		TF mRNA	TF protein	Surface TF activity	MV-TF from resting cells	MV-TF from activated cells	Increase in MV-TF activity	PAR2 mRNA	PAR2 protein	Time-point of maximal TF release
TF mRNA	Pearson Correlation	-	0.532*	0.097	0.104	0.717^**	0.341	0.745^**	0.103	0.342
	Significance		0.028	0.710	0.691	0.001	0.181	0.001	0.695	0.179
TF protein	Pearson Correlation	0.532*	-	−0.175	0.094	0.507*	0.441	0.548*	0.341	0.501*
	Significance	0.028		0.503	0.720	0.038	0.077	0.023	0.180	0.041
Surface TF activity	Pearson Correlation	0.097	−0.175	-	0.209	0.160	−0.196	0.119	0.186	0.391
Surface TF activity	Significance	0.710	0.503		0.420	0.539	0.451	0.650	0.475	0.121
MV-TF from resting cells	Pearson Correlation	0.104	0.094	0.209	-	0.483^*	−0.453	0.033	−0.238	0.030
MV-TF from resting cells	Significance	0.691	0.720	0.420		0.050	0.053	0.899	0.358	0.908
MV-TF from activated cells	Pearson Correlation	0.717^**	0.507*	0.160	0.483^*	-	0.370	0.770^**	0.242	0.484*
MV-TF from activated cells	Significance	0.001	0.038	0.539	0.050		0.144	<0.001	0.350	0.049
Increase in MV -TF Activity	Pearson Correlation	0.341	0.441	−0.196	−0.453	0.370	-	0.601^*	0.714^**	0.383
Increase in MV -TF Activity	Significance	0.181	0.077	0.451	0.053	0.144		0.011	0.001	0.129
PAR2 mRNA	Pearson Correlation	0.745^**	0.548*	0.119	0.033	0.770^**	0.601^*	-	0.309	0.618*
PAR2 mRNA	Significance	0.001	0.023	0.650	0.899	<0.001	0.011		0.227	0.008
PAR2 protein	Pearson Correlation	0.103	0.341	0.186	−0.238	0.242	0.714^**	0.309	-	0.430
PAR2 protein	Significance	0.695	0.180	0.475	0.358	0.350	0.001	0.227		0.085
Time-point of maximal TF release	Pearson Correlation	0.342	0.501*	0.391	0.030	0.484*	0.383	0.618*	0.430	-
Time-point of maximal TF release	Significance	0.179	0.041	0.121	0.908	0.049	0.129	0.008	0.085

Parameters associated with seventeen cell lines (shown on the table) were determined as shown in Figs. 1–4. Correlations between the measured cellular attributes were examined by performing multiple Pearson’s correlation analysis (n = 3; * = p < 0.05; ** = p < 0.01)

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