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Fig. 1 | Thrombosis Journal

Fig. 1

From: Hemophilia A gene therapy via intraosseous delivery of factor VIII-lentiviral vectors

Fig. 1

GFP expression in BM cells following IO infusion of M-GFP-LV. a Schematic of IO infusion of vectors into the mice with an infusion speed of 10 μl/min, which was precisely controlled by a programmable microfluidics syringe pump. b C57BL/6 mice were intraosseously delivered with M-GFP-LV (1.1 × 108 ifu/animal, n = 6) on day 0. BM cells were isolated from treated or untreated legs and GFP expression in LinSca1+c-Kit+ HSCs were examined on day 7 by flow cytometry. c C57BL/6 mice were given IO infusion of M-GFP-LV (1.1 × 108 ifu; n = 8) on day 0. GFP expression in LinSca1+c-Kit+ HSCs of treated and untreated legs was evaluated on day 124. d C57BL/6 mice were given IO infusion of M-GFP-LV (8.8 × 108 ifu/animal; n = 10) or PBS (20 μl/animal, mock; n = 5) on day 0. Long-term GFP expression in LinSca1+c-Kit+ HSCs was detected on day 160. This figure is reproduced from Ref [10]

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