Fig. 1
From: Reduced platelet hyper-reactivity and platelet-leukocyte aggregation after periodontal therapy
Flow cytometric features of leukocytes, neutrophils, monocytes, and lymphocytes and their complexes with platelets. Panel a is showing all events. The Neutrophils gate is characterized by high side scatter and low CD45 expression, the Monocytes gate is characterized by intermediate side scatter and intermediate CD45 expression, and the Lymphocyte gate is characterized by low side scatter and high CD45 expression. All events within the Neutrophils gate in a were represented in a CD66b and CD61 plot. Events characterized by high expression of both CD66b and CD61 designate the platelet-neutrophil complexes (PNCs, dotted box in b. Similar strategy was employed for the monocytes (using CD14, the platelet-monocyte complexes, PMCs, are in the dotted box in c and lymphocytes (using CD4, the platelet-lymphocyte complexes, PLymCs, are in the dotted box in d. An example of an unstimulated sample (incubated in HEPES buffer) is shown in b, c, and d, whereas flow cytometric analysis of a stimulated sample (incubated in ADP) is shown in e, f, and g