Fig. 2

Decreased concentrations of protein C and antithrombin are associated with decreased and increased APC generation, respectively. a Plasma samples with 0, 10, 30, 50, 70, and 100% protein C levels were prepared by the addition of vehicle, 0.36, 1.08, 1.81, 2.53, and 3.61 μg/mL of protein C, respectively, to protein C deficient plasma. b Plasma samples with 0, 10, 30, 50, 70, and 100% antithrombin levels were prepared by the addition of vehicle, 0.07, 0.25, 0.43, 0.61, and 0.88 U/mL of antithrombin, respectively, to antithrombin deficient plasma. These plasma samples were incubated with 0.2 U/mL of thrombin (T1063, Sigma-Aldrich, St. Louis, MO) and 1000 ng/mL of rTM at 37 °C for 10 min. APC levels were analyzed by the OLIGOBIND APC activity assay. The data represent mean ± SD (n = 3). Representative data of two independent experiments are shown. PNP: pooled normal plasma, PC: protein C, AT: antithrombin