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Fig. 2 | Thrombosis Journal

Fig. 2

From: The role of Sphingomyelin synthase 2 (SMS2) in platelet activation and its clinical significance

Fig. 2

SMS2 deficiency reduced platelet activation in aggregation, spreading and clot retraction experiments. a, b Platelets from WT and SMS2 –/– mice were re-suspended with Tyrode’s buffer. Thrombin- and collagen-induced aggregation were recorded in a platelet aggregator (black, WT, red, SMS2 –/–, n = 4, n.s. P > 0.05, * P < 0.05, *** P < 0.001). c, d WT and SMS2 –/– platelets spreading on fibrinogen-coated slides. Spreading areas were quantified at indicated time points (n = 4, ** P < 0.01). e, f WT and SMS2 –/– platelets were stimulated with thrombin (0.2 U/mL) to coagulate. Images were pictured at indicated timepoints (n = 4, ** P < 0.01). g, h Thrombin- and collagen-induced human platelet aggregation by adding D609 (red curve, 100 µM, purple curve, 30 µM, gray curve, 10 µM, black curve, DMSO control, n = 4, ** P < 0.01, *** P < 0.001). i, j Human platelets spreading on fibrinogen with or without D609 (100 µM). Fully spread ratios were quantified (n = 4, *** P < 0.001). k, l Washed platelets added with thrombin (0.2 U/mL) and D609 at indicated concentration to coagulate (red, 100 µM, gray 10 µM, black, DMSO control, n = 4, n.s. P > 0.05, ** P < 0.01, *** P < 0.001)

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