Fig. 3

Relative protein S (PROS1) mRNA and protein expression. (a) Relative green fluorescent protein (GFP) expression of cells transfected with mock, wild type (PROS1-WT), and the p.Leu607Ser mutation (PROS1-M) shows that there is no significant difference in transfection efficiency between the three groups. (b) Relative PROS1 mRNA expression of mock, PROS1-WT, and PROS1-M in HEK293T cells with glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as an internal reference. (c) Relative PROS1 mRNA expression of mock, PROS1-WT, and PROS1-M in HEK293T cells using GFP as an external reference. (d) PROS1 expression of mock, PROS1-WT, and PROS1-M in the supernatant and lysate of HEK293T cells, as detected by western blot. (e) According to the enzyme-linked immunosorbent assay (ELISA) standard curve, the expression of PROS1 mock, PROS1-WT, and PROS1-M in the supernatant of HEK293T cells is calculated. (f) According to the ELISA standard curve, the expression of PROS1 mock, PROS1-WT, and PROS1-M in HEK293T cell lysate is calculated. *p<0.05,** p<0.01,*** p<0.005, n = 3