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Fig. 3 | Thrombosis Journal

Fig. 3

From: The disulfide bond Cys2724-Cys2774 in the C-terminal cystine knot domain of von Willebrand factor is critical for its dimerization and secretion

Fig. 3

Expression of recombinant VWF protein in HEK-293T cells. A The results of genetic sequencing showed a G >A mutation at position 8171 of VWF. B Western blotting analysis of concentrated lysate and supernatant from cultured cells transfected with the plasmids of VWF WT, VWF C2724Y, and mixture of VWF WT and VWF C2724Y. Protein in the lysate and supernatant was separated by 8% reducing SDS-PAGE and immunoblotted with anti-VWF antibody

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Thrombosis Journal

ISSN: 1477-9560

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