Fig. 4

Multimer analysis of recombinant VWF protein in HEK-293T cells. Multimer analysis of concentrated lysate (A) and supernatant (B) of cultured cells transfected with the plasmid of vector, VWF WT, VWF C2724Y, and mixture of VWF WT and VWF C2724Y (1:2). Protein in the lysate and culture supernatant was separated by non-reducing electrophoresis on a 1.5% agarose gel and immunoblotted with anti-VWF antibody. NHP, normal human plasma