Fig. 4

GPVI shedding in manually mixed and agitated PCs during storage. A demonstrates representative western blot image illustrating shedding patterns of GPVI in one-day stored MM-PCs and CAG-PCs (in presence and absence of PP2 treatment). Graph B also compares GPVI shedding of those mentioned products (n = 3). Graph C compares different levels of GPVI shedding, detected by ELISA method among MM-PCs, CAG-PCs and PP2-treated CAG-PCs (n = 10) during storage. To better demonstrate significant differences between MM-PCs and CAG-PCs, the ratio of GPVI shedding to GPVI expression for each product were calculated and compared (D). CAG = continuously-agitated; MM = manually-mixed; .PC = platelet concentrate. Note: ns: not significant; *p < 0.05; **p < 0.01, ***p < 0.001