Fig. 5

Dual staining of GPVI expression and P-selectin in one-day stored manually mixed and agitated PCs. In order to confirm the previous findings that indicate different levels of GPVI in MM- and CAG-PCs with the same levels of P-selectin in these products, simultaneous staining of these receptors was performed one-day stored PCs. A shows a representative dotted plots of double stained platelets with FITC-anti P-selectin and PE-anti GPVI in either MM-PC or CAG-PCs (one-day stored PCs). B and C demonstrate representative histograms and their corresponding dotted plot for each of P-selectin and GPVI expression separately. (n = 10); CAG = continuously-agitated; MM = manually-mixed; .PC = platelet concentrate