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Fig. 3 | Thrombosis Journal

Fig. 3

From: Complement C5a induces the generation of neutrophil extracellular traps by inhibiting mitochondrial STAT3 to promote the development of arterial thrombosis

Fig. 3

Promotion of NETosis by C5a was Mito-ROS-dependent. A Fluorescence images showing ROS production in mitochondria indicated by MitoSox Red dye. Scale bar = 100 µm. B Quantification of the production of Mito-ROS shown as MitoROS relative fluorescence (folds of the control group) (n = 4 each). C Representative images of immunofluorescence staining for DNA (SYTOX green), CitH3 (red), Ly6g (blue) in vitro showing the presence of NETs. Scale bar = 75 µm. D Quantification of NET formation capacity shown as percentage of NET release in vitro, as assessed by immunofluorescence staining (n = 3 each). E NETosis was measured using a plate reader assay, (n = 3 each). Data are presented as mean ± SD

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