Clinical significance of neutrophil extracellular traps biomarkers in thrombosis

Thrombosis Journal

Table 3 Studies evaluating NETs biomarkers in IS

First author/year	Study design	Included patients	Groups (No. patients)	Samples processing	NETs biomarkers	Analytical methods for NETs biomarkers	Detailed values
O'Connell et al (2017) [86]	Case–control	Patients experiencing AIS and those identified as stroke mimics	AIS (n = 43) vs. Negative AIS (n = 20)	Plasma, 2000 × g, 10 min and 10,000 × g, 10 min	DNA	qPCR	NA
Vallés et al (2017) [87]	Case–control and cohort	Patients with AIS during the acute phase of brain ischemia and suffering stroke < 24 h before admission	AIS (n = 243) vs. HC (n = 27)	Plasma, 22 °C, 2500 × g, 10 min	H3Cit	ELISA	0.080 vs. 0.039 AU
					Nucleosomes	ELISA	0.329 vs. 0.209 AU
					DNA	SytoxGreen fluorimetry	432.11 vs. 324.2 ng/mL
Lim et al (2020) [75]	Case–control and cohort	Patients with newly diagnosed ACS or AIS	ACS (n = 37) vs. AIS (n = 58) vs. HC (n = 25)	Plasma, 1600 × g, 15 min	DNA-histone	ELISA	19.73 vs. 13.71 vs. 14.32 mU
Lim et al (2020) [75]	Case–control and cohort	Patients with newly diagnosed ACS or AIS	ACS (n = 37) vs. AIS (n = 58) vs. HC (n = 25)	Plasma, 1600 × g, 15 min	DNA	PicoGreen fluorimetry	743.28 vs. 524.22 vs. 216.48 ng/mL

Abbreviations: ACS Acute coronary syndrome, AIS Acute ischemic stroke, AU Absorbance unit, H3Cit Citrullinated histone H3, ELISA Enzyme-linked immunosorbent assay, HC Healthy control, IS Ischemic stroke, Min Minute, NA Not available, NETs Neutrophil extracellular traps, qPCR Quantitative polymerase chain reaction

ISSN: 1477-9560