Table 1 PCR Conditions & Primers Sequence for VKORC1 and CYP2C9 Genotyping
polymorphism or allele | Primers sequence | Product size (bp) | Annealing TM (C°) | Cycles |
|---|---|---|---|---|
-1639G > A | F: 5'-GCCAGCAGGAGAGGGAAATA-3' R: 5'-AGTTTGGACTACAGGTGCCT-3' | 290 | 60 | 35 |
*2,*8, *14,*20,*26, *33, *40, *41, *42, *43, *45, *46, *62, *63, *72, *73, *78 | F: 5'-TACAAATACAATGAAAATATCATG-3' R: 5'-CTAACAACCAGGACTCATAATG-3' | 690 | 46 | 40 |
*3,*4, *5, *24, *55, *66,*68 | F: 5'-TGCACGAGGTCCAGAGATAC-3' R: 5'-ACAAACTTACCTTGGGAATGAGA-3' | 105 | 53 | 35 |