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Fig. 2 | Thrombosis Journal

Fig. 2

From: Identification and characterization of two SERPINC1 mutations causing congenital antithrombin deficiency

Fig. 2

Gene cloning and plasmid vector construction. (a) Schematic diagram of mutant c.1377delC plasmid vector: pCDH-CMV-SERPINC1 (c.1377delC) -EF1-copGFP-T2A-Puro. (b) Schematic diagram of mutant c.685 T plasmid vector: pCDH-CMV-SERPINC1 (c.685C > T) -EF1-copGFP-T2A-Puro. (c) Electropherogram of wild-type plasmid digested with NotI/XhoI; (d) Electropherogram of mutant c.1377delC plasmid digested with NotI/XhoI; (e) Electropherogram of mutant c.685C > T plasmid digested with NotI/XhoI, Lane 1: undigested electropherogram, Lane 2: electropherogram after double digestion, Lane 3: DNA marker. (g-j) Sequencing of the clone plasmid of the SERPINC1 gene

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Thrombosis Journal

ISSN: 1477-9560

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