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Fig. 3 | Thrombosis Journal

Fig. 3

From: Identification and characterization of two SERPINC1 mutations causing congenital antithrombin deficiency

Fig. 3

(a) Green fluorescent protein expression after transfection of cells carrying unloaded (Control), wild-type (SERPINC1-WT), Asn460Thrfs*20 mutant (SERPINC1-c.1377delC), and Arg229* mutant (SERPINC1-685C > T), suggesting that there was no significant difference in transfection efficiency among the groups. (b) Relative SERPINC1 mRNA expression levels (*, P < 0.05, * *, P < 0.01, * *, P < 0.005, * *, P < 0.001) in HEK293T cells by Control, SERPINC1-WT, Asn460Thrfs20 mutant (SERPINC1-c.1377delC), and Arg229* mutant (SERPINC1-685C > T). (c) Western blotting was used to detect the expression of AT protein in HEK293T cells by Control, SERPINC1-WT, Asn460Thrfs*20 mutant (SERPINC1-c.1377delC) and Arg229* mutant (SERPINC1-685C > T), respectively. (d) Based on the enzyme-linked immunosorbent assay (ELISA) standard curve, the expression of Control, SERPINC1-WT, Asn460Thrfs*20 mutant (SERPINC1-c.1377delC), and Arg229* mutant (SERPINC1-685C > T) in the supernatant of HEK293T cells was calculated. (*, P < 0.05, * *, P < 0.01, * *, P < 0.005, * *, P < 0.001)

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Thrombosis Journal

ISSN: 1477-9560

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