Fig. 2From: LINC00659 exacerbates endothelial progenitor cell dysfunction in deep vein thrombosis of the lower extremities by activating DNMT3A-mediated FGF1 promoter methylationLINC00659 inhibits the proliferation, migration, and angiogenesis ability of EPCs. EPCs were transfected with pcDNA-LINC00659 or LINC00659 siRNA alone for 24 h. A RT-qPCR detection of the LINC00659 expression level of EPCs transfected with pcDNA-LINC00659 or LINC00659 siRNA (n = 6). B EPC, transfected with pcDNA-LINC00659 or LINC00659 siRNA, proliferation was checked by CCK-8 assay (n = 6). C Transwell assay was applied to test the effects of pcDNA-LINC00659 or LINC00659 siRNA transfected on EPC migration (n = 6). D Tube formation assay was applied to test the effects of pcDNA-LINC00659 or LINC00659 siRNA transfected on EPC angiogenesis (n = 6). E–F Effect of pcDNA-LINC00659 or LINC00659 siRNA transfected on actin cytoskeleton structure in cultured EPCs (n = 4). Each experiment was repeated for 3 times. **P < 0.01Back to article page