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Fig. 3 | Thrombosis Journal

Fig. 3

From: LINC00659 exacerbates endothelial progenitor cell dysfunction in deep vein thrombosis of the lower extremities by activating DNMT3A-mediated FGF1 promoter methylation

Fig. 3

LINC00659 upregulated EIF4A3 expression. A The consensus sequence of EIF4A3 motif, and the possible binding sites of LINC00659 and EIF4A3. B The relationship between LINC00659 and EIF4A3 in EPCs (n = 6). C Luciferase reporter assay investigated whether LINC00659 could bind to the EIF4A3 promoter at the putative sites (n = 6). D The enrichment of LINC00659 in the EIF4A3 precipitate was determined by RIP (n = 6). E RT-qPCR displayed the EIF4A3 mRNA expression with pcDNA-LINC00659 or LINC00659 siRNA transfection (n = 6). F Western blotting displayed the EIF4A3 protein level with pcDNA-LINC00659 or LINC00659 siRNA transfection (n = 6). G Western blotting detection of EIF4A3 level in EPCs isolated from patients with LEDVT and healthy controls (n = 6). Each experiment was repeated for 3 times. ***P < 0.001, **P < 0.01.

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