Skip to main content
Fig. 4 | Thrombosis Journal

Fig. 4

From: LINC00659 exacerbates endothelial progenitor cell dysfunction in deep vein thrombosis of the lower extremities by activating DNMT3A-mediated FGF1 promoter methylation

Fig. 4

EIF4A3 overexpression reversed the promoting effect of LINC00659 interference on EPCs migration and angiogenesis. LINC00659 siRNA was transfected alone or together with pcDNA-EIF4A3 into EPCs for 24 h. A Western blotting was employed to determine the levels of EIF4A3 in EPCs transfected with LINC00659 siRNA or/and pcDNA-EIF4A3 (n = 6). B The cell proliferation of EPCs transfected with LINC00659 siRNA or/and pcDNA-EIF4A3 was checked by CCK-8 assay (n = 6). C Transwell detection of the cell migration of EPCs transfected with LINC00659 siRNA or/and pcDNA-EIF4A3 (n = 6). D Tube formation assay was applied to test the effects of LINC00659 siRNA or/and pcDNA-EIF4A3 on EPC angiogenesis (n = 6). E–F Effect of LINC00659 siRNA or/and pcDNA-EIF4A3 on actin cytoskeleton structure in cultured EPCs (n = 4). Each experiment was repeated for 3 times. *P < 0.05, **P < 0.01

Back to article page

Thrombosis Journal

ISSN: 1477-9560

Contact us