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Fig. 5 | Thrombosis Journal

Fig. 5

From: LINC00659 exacerbates endothelial progenitor cell dysfunction in deep vein thrombosis of the lower extremities by activating DNMT3A-mediated FGF1 promoter methylation

Fig. 5

EIF4A3 recruits DNMT3A to the FGF1 promoter region and represses FGF1 expression. A Co-IP was employed to check the interaction between EIF4A3 and DNMT3A (n = 6). B The enrichment of DNMT3A in the FGF1 promoter region was tested by ChIP assay (n = 6). C Western blotting showed the FGF1 protein expression with LINC00659 siRNA or/and pcDNA-EIF4A3 transfection (n = 6). D Methylation levels of the FGF1 promoter in EPCs were checked by MSP-PCR (n = 6). E Western blotting was applied to test the protein level of FGF1 in EPCs (n = 6). F Western blotting detection of FGF1 level in EPCs isolated from patients with LEDVT and healthy controls (n = 6). SGI-1027: DNMT3A inhibitor (8 µM). Each experiment was repeated for 3 times. *P < 0.05, **P < 0.01, ***P < 0.001

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Thrombosis Journal

ISSN: 1477-9560

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